Isolation of an Enzyme-Coenzyme A Intermediate from Succinyl Coenzyme A-Acetoacetate Coenzyme A Transferase
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چکیده
منابع مشابه
Purification and properties of succinyl-coenzyme A-3-oxo acid coenzyme A-transferase from sheep kidney.
CoA-transferase (succinyl-CoA-3-oxo acid CoA-transferase, EC 2.8.3.5) isolated from sheep kidney was purified to homogeneity. The purified enzyme has a specific activity of approx. 200 units/mg. A mol.wt. of 110000 was obtained by gel filtration on Sephadex G-200, and a lower mol.wt. of 102000 was determined by analytical ultracentrifugation. A sedimentation coefficient of 5.6S was also determi...
متن کاملRegulation of Succinyl Coenzyme A:Acetoacetyl Coenzyme A Transferase in Rat Hepatoma Cell Lines1
The regulation of succinyl-CoA:acetoacetyI-CoA transferase (CoA transferase) has been studied in 8 rat hepatoma cell lines. Compared with normal rat hepatocytes, which have almost nondetectable activity of the enzyme, the hepatoma cell lines have a wide range of expression of CoA transferase activity, from as low as 45 nmol/min/mg to as high as 960 nmol/min/mg. \Yestern blotting showed that the...
متن کاملRegulation of succinyl coenzyme A:acetoacetyl coenzyme A transferase in rat hepatoma cell lines.
The regulation of succinyl-CoA:acetoacetyl-CoA transferase (CoA transferase) has been studied in 8 rat hepatoma cell lines. Compared with normal rat hepatocytes, which have almost nondetectable activity of the enzyme, the hepatoma cell lines have a wide range of expression of CoA transferase activity, from as low as 45 nmol/min/mg to as high as 960 nmol/min/mg. Western blotting showed that the ...
متن کاملSubcellular localization of acetoacetate coenzyme A transferase in rat hepatomas.
Succinyl coenzyme A:acetoacetate coenzyme transferase (EC 2.8.3.5), an initiator of ketone body usage and absent in normal liver, has been shown to be located in mitochondria from Morris hepatoma 7288ctc using differential and density gradient centrifugation. Furthermore, tumor mitochondrial subfractionation revealed that this transferase is associated with the matrix-soluble proteins. Comparis...
متن کاملIsolation of coenzyme A.
The coenzyme content was followed throughout the purification by the assay method described in a preceding paper (2). Hog liver was found most suitable. Ten 50 pound batches of liver were used in this preparation. Since rapid autolysis of Co A occurred, the yield was largely dependent on the rapidity with which the livers were obtained. Livers from still warm hog bodies were cut into small port...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)81470-9